Thermo Fisher, 10360014, Bac-to-Bac™ Vector Kit

Quantity: 1 kit
Unit Size: Each
• Contains pFastBac™1 vector, as well expression control vector, intended for use as part of Bac-to-Bac Baculovirus Expression System, which enables efficent production of recombinant baculovirus for expression testing in insect cells
• Relies on generation of recombinant baculovirus by site-specific transposition inE. colirather than homologous recombination in insect cells
• pFastBac 1 vector provided by this kit offers strong polyhedrin promoter for protein expression and large multiple cloning site for simplified cloning
• Time-saving expression bacmid: expression cassette of pFastBac vector recombines with parent bacmid in DH10BacE. coli
• Competent Cells to form expression bacmid: bacmid is then transfected into insect cells for production of recombinant baculovirus particles
• Easy colony screening
• Parent bacmid in DH10BacE. colicontains segment oflacZα gene
• lacZα gene is disrupted upon transposition of expression cassette into bacmid allowing for blue/white selection of recombinantsBaculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis