Thermo Fisher, 10748010, BenchMark™ Pre-stained Protein Ladder

Name: BenchMark™ Pre-stained Protein Ladder
Brand: Thermo Fisher
SKU: 10748010

Quantity: 2 x 250 μL, Unit Size: Each

Catalog Number: 10748010

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Category

TagsProtein Molecular Weight Markers Gel Electrophoresis Equipment and Supplies Gel Electrophoresis Reagents and Buffers

CollectionsThermo Fisher Gel Electrophoresis: Agarose Gels, SDS-PAGE & Blotting Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 2 x 250 μL
Unit Size: Each
The BenchMark Pre-Stained Protein Ladder consists of 10 proteins ranging in apparent molecular weight from 6 to 180 kDa designed for use with Tris-Glycine gels.
• Proteins covalently bound to a blue dye for easy visualization
• One protein band coupled to a pink dye for easy orientation and protein identification
• Affinity-purified proteins to provide sharp bands and clear background
• Easy-to-use format for direct application to an SDS-PAGE gel
• Sufficient reagent for 100 mini gels or 50 regular gels following Western transfer
• Sufficient reagent for 50 mini-gels or 25 regular gels during electrophoresis
• Gel Compatibility: NovexTris-Glycine Gels1D Gel Electrophoresis, Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis, Western Blotting

Specifications

General

Detection Method	Colorimetric
Gel Compatibility	Novex™ Tris-Glycine Gels
Molecular Weight (g/mol)	180, 115, 82, 64, 49, 37, 26, 19, 15, 6 kDa
Quantity	2 x 250 μL
Ready to Load	Yes
Shipping Condition	Dry Ice
Number of Markers	10
Product Line	BenchMark
Product Type	Protein Ladder
Size Range	6 to 180 kDa
Stain Type	Blue, Pink
System Type	Western Blotting, SDS-PAGE
Unit Size	Each

FAQs

Question: What molecular-weight range does the ladder cover?

Answer:It contains ten bands spanning approximately 6–180 kDa.

Question: How is the orientation band identified?

Answer:The fourth band from the top is pink while the other bands are blue.

Question: How much ladder is supplied?

Answer:The package contains two 250 µL vials.

Application Note

BenchMark™ Pre-Stained Protein Ladder is a ready-to-use molecular-weight marker for monitoring SDS-PAGE separation and western-transfer performance. The ladder contains ten prestained protein bands spanning approximately 6–180 kDa. Most bands are blue, while the fourth band from the top is pink, providing an easy visual orientation point during electrophoresis and membrane transfer. This configuration contains two 250 µL vials. Covalently attached dyes allow the bands to remain visible during the run and after transfer, helping confirm migration and transfer efficiency without staining the gel or membrane first. Prestaining alters apparent migration compared with unstained standards, so the ladder is best suited to approximate molecular-weight estimation and process monitoring rather than high-accuracy mass determination. Store the ladder frozen at approximately −30 to −10 °C and mix gently before loading.

Ordering

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