Thermo Fisher, 12322012, PureLink™ Plant RNA Reagent

Name: PureLink™ Plant RNA Reagent
Brand: Thermo Fisher
SKU: 12322012

Quantity: 100 mL, Unit Size: Each

Catalog Number: 12322012

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Category

RNA Extraction Kits

Pack Size100 mL

TagsMolecular Biology Reagents and Kits RNA Isolation and Purification Products

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 mL
Unit Size: Each
Plant RNA Reagent is a single-component solution optimized for the isolation of high yields of high-quality total RNA from a variety of plant tissues, especially those rich in polyphenolics (e.g., conifer needles) or starch (e.g., potato tuber or seeds).Improved quality and purity of plant RNA Plant RNA Reagent is optimized to have less sample DNA contamination, to be twice as effective as TRIzol Reagent, and have a simplified RNA extraction protocol for minimized time and labor. The end result is high-yield, high-purity RNA (determined by A_260/A_280 and gel analysis) from a variety of plant material sources.Works for milligram to gram quantities of starting materialPlant RNA Reagent provides greater flexibility for sample size and processing formats, and can be used for both small-scale (up to 0.1 g plant tissue) and large-scale (0.1–5 g plant tissue) total RNA isolation. The amount supplied (100 mL) is sufficient for 200 reactions using 100 mg of tissue, or 4 reactions using 5g of tissue.
• Isolate high-quality RNA from difficult plant materials
• Use for small-scale and large-scale RNA isolations
• Fewer resources used, less hazardous, less wasteIsolated RNA is ideal for a variety of applications and products:
• cDNA library construction kits
• Real-Time PCR (qPCR)
• Northern blotting kits and reagents
• Poly(A) selection kit RNase control reagents and assaysNot high-throughput compatible (Manual)

Specifications

General

Final Product Type	Total RNA
For Use With (Application)	Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Northern Blotting
High-throughput Compatibility	Not High-throughput Compatible (Manual)
No. of Reactions	200 Preps
Purification Time	1 hr.
Quantity	100 mL
Scale	Scaleable
Starting Material Amount	Up to 1 g tissue, Up to 1 x 10^7 cells
Isolation Technology	Organic Extraction
Sample Type	Plant Samples
Target	Total RNA
Unit Size	Each

FAQs

Question: What quantity is supplied?

Answer:A 100 mL bottle of PureLink Plant RNA Reagent.

Question: Which samples are suitable?

Answer:Plant tissues, including starch-rich and polyphenol-rich specimens.

Question: What can the isolated RNA be used for?

Answer:Examples include RT-PCR, qPCR, Northern blotting, and cDNA library preparation.

Application Note

PureLink™ Plant RNA Reagent is a ready-to-use solution for isolating total RNA from plant tissues, including difficult samples rich in polyphenols or starch. It supports extraction from materials such as conifer needles, potato tubers, and seeds while simplifying sample preparation for downstream molecular biology. This 100 mL reagent configuration is described for approximately 200 preparations using 100 mg of plant tissue, with the usable number of preparations depending on input mass and protocol scale. Purified RNA can support reverse transcription PCR, quantitative PCR, Northern blotting, cDNA library construction, and other gene-expression analyses. The reagent uses organic extraction chemistry and is intended for manual processing rather than a prefilled automated instrument cartridge. Store the solution at 4°C.

Ordering

Notice

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