Thermo Fisher, 12538120, LR Clonase™ II Plus enzyme

Name: LR Clonase™ II Plus enzyme
Brand: Thermo Fisher
SKU: 12538120

Quantity: 20 Reactions, Unit Size: Each, Gateway™ LR Clonase™ enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR sites) to generate your expression clone. We offer different formats of LR Clonase enzyme mixtures, depending on your application and desired format.

Catalog Number: 12538120

Pricing

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Category

Seamless Cloning Reagents

Pack Size20 reactions

TagsModifying Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 20 Reactions
Unit Size: Each
LR Clonase II Plus enzyme mix is the latest version that offers the highest recombination efficiency available(Table 1), and is specifically optimized for both single and multiple fragment cloning (Table 1). LR Clonase II Plus enzyme mix is provided in an optimized single mix of enzyme and reaction buffer, ensuring enzyme stability and easy of use with few pippetting steps for both single and multiple fragment cloning. Our LR Clonase II enzyme mix is also available in a single mix format, while the original LR Clonase® enzyme is the enzyme and buffer in separate tubes.

Specifications

General

Compatible Buffer	Reaction Buffer
Product Type	LR Clonase Enzyme
Quantity	20 Reactions
Shipping Condition	Dry Ice
Enzyme	LR Clonase
Product Line	Clonase, Gateway
Unit Size	Each

FAQs

Question: How many reactions are included?

Answer:This configuration supports 20 reactions.

Question: What type of cloning is the Plus formulation optimized for?

Answer:It is optimized for both single-fragment and multi-fragment Gateway LR recombination.

Question: Which recombination proteins are included?

Answer:The enzyme formulation includes Integrase, Excisionase, and Integration Host Factor.

Application Note

Gateway™ LR Clonase™ II Plus Enzyme Mix is an optimized recombination reagent for transferring attL-flanked entry-clone inserts into attR-containing destination vectors. This configuration supports 20 reactions and is formulated for both single-fragment and multi-fragment Gateway cloning. The mix contains bacteriophage lambda Integrase and Excisionase together with E. coli Integration Host Factor and the required reaction buffer in one formulation. It is used to generate attB-containing expression clones and is particularly suited to MultiSite Gateway workflows in which multiple entry clones are recombined in a defined order. Proteinase K solution is included for post-reaction treatment. The optimized Plus formulation provides a streamlined alternative when high recombination efficiency or multi-fragment assembly is required.

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
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