Thermo Fisher, 15154, Pierce™ Protein Coated Plates

Name: Pierce™ Protein Coated Plates
Brand: Thermo Fisher
SKU: 15154

Color: White, Format: Standard, Surface Treatment: Protein A, Unit Size: Each, These plates are uniformly and stably coated with one of four popular immunoglobilin-binding proteins (Protein A, Protein G, Protein A/G, or Protein L). They enable antibodies, or pre-established antibody-antigen complexes, to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-t

Catalog Number: 15154

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Category

General Purpose Solvents

TagsMicroplates Coated Binding Assay Microplates

CollectionsThermo Fisher Microplates for ELISA, Fluorescence & Imaging Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Color: White
Format: Standard
Surface Treatment: Protein A
Unit Size: Each
Thermo Scientific Pierce Protein Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.Highlights• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption• Orient antibodies for maximum antigen-binding capacity• Immobilize antibodies for plate assays without prior purification• Ensure minimal variation (<5% well-to-well) from consistent coating• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)Protein A/G Coated Plates• Fusion protein containing four antibody-binding sites from Protein A and two from Protein G• Binds to most IgG species because it combines the specificities of Protein A and Protein G• Binds to Fc region of antibodies, ensuring optimal orientation• Binds well using a wide range of pHProtein A Coated Plates• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog, and rhesus monkey• Binds strongly to mouse IgG2a, IgG2b and IgG3• Binds to Fc region of antibodies for optimal orientationProtein G Coated Plates• Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat• Binds only to IgG—no cross-reactivity with other antibody classes• Binds to Fc region of antibodies for optimal orientation Protein L Coated Plates• Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)• Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites• Binds to Fab fragments and ScFv• Does not bind bovine, goat or sheep immunoglobulin• Binds weakly to rabbit immunoglobulin• Pre-blocked to reduce nonspecific binding

Specifications

General

Binding Property	Standard Capacity, Affinity
Detection Method	Chemiluminescence
No. per Pack	5 Plates
Plate Blocking	SuperBlock™ Buffer
Product Type	Microplate
Quantity	5 plates
Color	White
Format	Standard
Material	Polystyrene
No. of Wells	96
Product Line	Pierce
Surface Treatment	Protein A
Unit Size	Each

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