Thermo Fisher, 18009027, CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/μL

Quantity: 1000 U
Unit Size: Each
• Concentration: 20 units/μL
• Source: Purified from calf intestinal mucosa
• Performance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay
• Unit Definition: One unit hydrolyzes 1μmol of 4-nitrophenyl phosphate in 1 min. at 37°C
• Unit Reaction Conditions: 1M diethanolamine buffer, 10mM 4-nitrophenyl phosphate, 0.25mM MgCl_2(pH 9.8) in 900μL for 10 min. at 37°CDephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning