Thermo Fisher, 18021071, Ribonuclease H, 120 U

Quantity: 120 U
Unit Size: Each
• Source: Purified fromE. coliexpressing theE. coliRNase H gene on a plasmid
• Performance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease
• Unit definition: One unit hydrolyzes 1nmol of RNA in^3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min. at 37°C
• Unit reaction conditions: 20mM Tris-HCl (pH 7.5), 0.1M KCl, 10mM MgCl_2, 0.1mM DTT, 5% (w/v) sucrose, 0.5nmol^3H-labeled poly(A)poly(dT), and enzyme in 50μL for 20 min. at 37°CRemoval of mRNA during second-strand cDNA synthesis; Removal of poly(A) sequences from mRNA in the presence of oligo(dT); Oligodeoxyribonucleotide-directed cleavage of RNA; PCR & Real-Time PCR; Reverse Transcription