Thermo Fisher, 18068015, DNase I, Amplification Grade

Quantity: 100 U
Unit Size: Each
DNase I, Amplification Grade, digests single- and double-stranded DNA to oligodexyribonuleotides containing a 5' phosphate. DNase I, Amplification Grade, is suitable for eliminating DNA during critical RNA purification procedures such as those prior to RNA-PCR amplification. Absence of RNase is tested by performing a ribonuclease assay with RNA ladder.
• Specific activity is >10,000 units/mg
• Source: Purified from bovine pancreas
• Performance and quality testing: Ribonuclease assay with RNA ladder and ability to digest single-stranded and double-stranded DNA to oligonucleotides are determined
• Unit definition: One unit increases absorbance of high molecular weight DNA solution at rate of 0.001 A_260units/min./mL of reaction mixture at 25°C
• Unit reaction conditions: 0.1M sodium acetate (pH 5.0), 5mM MgCl_2, 50 μg/mL calf thymus DNA, and enzyme in 1mL for 10 min. at 25°CRemoving DNA from RNA and protein preparations, PCR & Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription