Thermo Fisher, 18095011, BioPrime™ Array CGH Genomic Labeling System

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Using the BioPrime Plus Array CGH Genomic Labeling System, you can expect:––High yields of fluorescently labeled genomic DNA–signal-to-noise ratios–detection of gene copy number variations.These systems combine a highly concentrated exo-Klenow fragment of DNA polymerase I and random primers to effectively label genomic targets for sensitive genomic profiling experiments. Exo-Klenow is a mutant of the large fragment of the DNA polymerase I holoenzyme that has both 5' - 3' and 3' - 5' exonuclease activity removed. The lack of exonuclease activity makes this enzyme ideal for use in random priming protocols, enabling robust yields and efficient incorporation of Alexa Fluor™ AHA fluorescent nucleotides. In the BioPrime Plus Array CGH Genomic Labeling Systems, random octamers anneal to template DNA, providing priming sites for the exo- Klenow enzyme. Alexa Fluor AHA modified nucleotides (direct labeling) or amino-allyl modified nucleotides (indirect labeling) are incorporated as the polymerase extends from the priming sites. The labeled samples are then purified to remove contaminants prior to denaturing and hybridization to a microarray (direct labeling systems) or coupled to the Alexa Fluor NHS ester (indirect labeling systems) prior to hybridization. Available in a direct labeling format.Array CGH, ChIP-on-Chip, Chromatin Biology, DNA and RNA Purification and Analysis, DNA Labeling, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Nucleic Acid Labeling and Oligo Synthesis, RNAi, Epigenetics and Non-Coding RNA Research