Description
Quantity: 2 mg
Unit Size: Each
These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer. Highlights:
• Recombinant streptavidin– high-quality protein, 53kDa, pI equals 6.8 to 7.5
• Pure protein and enzyme conjugates– choose pure, unconjugated protein for biotin blocking and controls; enzyme conjugates for substrate-based detection
• Fluorophore conjugates– standard fluorescent forms (fluorescein, rhodamine, etc.) for traditional immunofluorescence techniques
• Activated preparations– choose a maleimide-activated or hydrazide-activated form to prepare a custom streptavidin conjugate with sulfhydryl- or carbohydrate-containing molecule