Thermo Fisher, 21875034, RPMI 1640 Medium, 500 mL
Quantity: 500 mL, Unit Size: Each
Catalog Number: 21875034
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Category
Pack Size500 mL
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| Cell Type | Mammalian Cells |
|---|---|
| Concentration | 1 X |
| Culture Environment | CO<sub>2</sub> |
| Green Features | Sustainable packaging |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Osmolality | 260 - 310 mOsm/kg |
| Product Line | Gibco |
| Product Type | RPMI 1640 |
| Quantity | 500 mL |
| Shelf Life | 12 Months |
| Shipping Condition | Room Temperature |
| Classification | Animal Origin-free |
| Culture Type | Mammalian Cell Culture |
| Form | Liquid |
| Serum Level | Standard Serum Supplementation |
| Sterility | Sterile-filtered |
| With Additives | Phenol Red |
| Without Additives | No HEPES |
| pH | 7 to 7.4 |
| Unit Size | Each |
Answer:One 500 mL bottle of ready-to-use sterile-filtered liquid RPMI 1640 medium.
Answer:L-glutamine, phenol red and sodium bicarbonate are included; HEPES and sodium pyruvate are not included.
Answer:No. Serum or another suitable supplement is commonly added separately as specified in the cell-culture protocol.
Gibco RPMI 1640 is a sterile-filtered liquid basal medium for mammalian cell culture, including suspension and adherent cells that are compatible with an RPMI formulation. This 500 mL bottle contains L-glutamine, phenol red and sodium bicarbonate; the exact formulation does not include HEPES or added sodium pyruvate. RPMI 1640 contains vitamins and glutathione and is commonly used for cell lines such as Jurkat cells and other hematopoietic cell models. The basal medium does not supply serum-derived growth factors, so suitable cultures require supplementation, often fetal bovine serum, at a level determined by the relevant protocol. Sodium bicarbonate buffering is intended for operation under approximately 5–10% CO₂. Store this liquid medium protected from light at 2–8 °C. Confirm the culture-specific formulation and avoid substituting other RPMI variants that differ in glutamine or phenol-red content.
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