Thermo Fisher, 12634028, Advanced DMEM/F-12, 10 x 500 mL
Quantity: 10 x 500 mL, Unit Size: Each
Catalog Number: 12634028
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Pack Size10 x 500 mL
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| Cell Line | MRC-5, SP2, Vero, WI-38 and Jurkat |
|---|---|
| Concentration | 1 X |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Product Line | Gibco |
| Product Type | DMEM (Dulbecco's Modified Eagle Medium)/F-12 |
| Quantity | 10 x 500 mL |
| Shelf Life | 12 Months From Date of Manufacture |
| Shipping Condition | Room Temperature |
| Classification | Animal Origin |
| Form | Liquid |
| Serum Level | Reduced Serum |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | High Glucose |
| Without Additives | No HEPES |
| Unit Size | Each |
Answer:Ten separate 500 mL bottles of sterile-filtered 1× Advanced DMEM/F-12 liquid medium.
Answer:No. Add 4 mM L-glutamine or an appropriate GlutaMAX supplement and optimize serum supplementation for the cell line.
Answer:Use a CO₂-controlled incubator compatible with bicarbonate buffering; refrigerate unopened medium at 2–8°C and protect it from light.
Gibco Advanced DMEM/F-12 is a ready-to-use 1× basal medium for mammalian cell culture under reduced-serum conditions. It combines the DMEM/F-12 nutrient background with insulin, transferrin, lipid-rich albumin and additional growth-supporting components. The liquid formulation contains glucose, sodium pyruvate, non-essential amino acids and phenol red but does not contain L-glutamine or HEPES. Ten separately packaged 500 mL bottles are supplied. For routine culture, supplement with approximately 1–5% fetal bovine serum and 4 mM L-glutamine or a suitable GlutaMAX alternative, then optimize the formulation for the cell line. The bicarbonate-buffered medium requires a controlled CO₂ atmosphere, typically 5–10%, to maintain physiological pH. The reduced-serum design may lower serum requirements compared with conventional DMEM/F-12, but performance depends on cell type and passage history. Store unopened bottles at 2–8°C protected from light and use aseptic technique when dispensing.
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