Thermo Fisher, 22108, SM(PEG)8 (PEGylated, long-chain SMCC crosslinker)

Name: SM(PEG)8 (PEGylated, long-chain SMCC crosslinker)
Brand: Thermo Fisher
SKU: 22108

Quantity: 100 mg, Unit Size: Each, Thermo Scientific™ Pierce SM(PEG)n is a series of amine-to-sulfhydryl crosslinkers that differ in length from 17.6 to 95.2 Ås as a result of polyethylene glycol spacer arms containing n equals 2 to 24 ethylene glycol units.

Catalog Number: 22108

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Category

Protein Crosslinkers

Pack Size100 mg

TagsProtein Analysis Reagents Crosslinking Labeling and Protein Modification Crosslinking Reagents

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 mg
Unit Size: Each
The SM(PEG)_n reagents are six crosslinkers that have identical amine- and sulfhydryl-reactive groups but differ in length as a result of spacer arms composed of different numbers of ethylene glycol units. Their structures (shown below) have the form NHS-(PEG)_n-Maleimide, where n represents 2, 4, 6, 8, 12 or 24 polyethylene glycol (PEG) units. Each SM(PEG)_n reagent is produced at the specified length without contamination from analogs of other lengths.The availability of this set of reagents makes it possible to optimize crosslinking experiments to obtain the best conjugate efficiency and function for the intended application or analysis method. Considered together with AMAS (Part No. 22295) and the popular SMCC (Part No. 22360), these reagents allow for amine-to-sulfhydryl crosslinking across spaces ranging from 4.4 to 95.2Å. In addition, the hydrophilic PEG content of SM(PEG)_n reagents adds to the aqueous solubility of these crosslinkers and their conjugates. PEG is also less immunogenic than other types of crosslinker spacers, making these reagents ideal for immunogen preparation. Highlights:
• Homologous series of increasing spacer arm length, ideal for optimizing conjugation of protein interactions
• Water-soluble polyethylene glycol (PEG) spacer arms help to maintain or increase solubility of crosslinked proteins
• Reagents readily dissolve in DMSO for subsequent dilution in aqueous reaction buffers
• Reactive groups:NHS ester and maleimide
• Reactive toward:amino and sulfhydryl groups NHS-PEG_2-Maleimide
• Two ethylene oxide subunits
• Alternate names: Mal-dPEG_2-NHS, NHS-PEG_2-Maleimide
• Molecular formula C_18H_23N_3O_9
• Spacer arm length 17.6Å
• Mass added to target per crosslink 310.12
• MW 425.39 NHS-PEG_4-Maleimide
• Four ethylene oxide subunits
• Alternate names Mal-dPEG_4-NHS, NHS-PEG_4-Maleimide
• Molecular formula C_22H_31N_3O_11
• Spacer arm length 24.6Å
• Mass added to target per crosslink 398.17
• MW 513.50& NHS-PEG_6-Maleimide
• Six ethylene oxide subunits
• Alternate names Mal-dPEG_6-NHS, NHS-PEG_6-Maleimide
• Molecular formula C_26H_39N_3O_13
• Spacer arm length 32.25Å
• Mass added to target per crosslink 486.20
• MW 601.60NHS-PEG_8-Maleimide
• Eight ethylene oxide subunits
• Alternate names Mal-dPEG_8-NHS, NHS-PEG_8-Maleimide
• Molecular formula C_30H_47N_3O_15
• Spacer arm length 39.2Å
• Mass added to target per crosslink 574.27
• MW 689.71NHS-PEG_12-Maleimide
• 12 ethylene oxide subunits Mal-dPEG_12-NHS, NHS-PEG_12-Maleimide
• Molecular Formula C_38H_63N_3O_19
• Spacer arm length 53.4Å
• Mass added to target per crosslink 750.38
• MW 865.92

Specifications

General

Cell Permeability	No
Description	SM(PEG)8
Form	Solid
Labeling Method	Chemical Labeling
Molecular Weight (g/mol)	689.71
PEGylated	Yes
Product Line	Pierce
Quantity	100 mg
Reactive Moiety	Maleimide, NHS Ester
Shipping Condition	Ambient
Solubility	Water
Spacer Arm Length	39.2 Å
Water Soluble	Yes
Chemical Reactivity	Amine-Sulfhydryl
Cleavable	No
Crosslinker Type	Heterobifunctional
Format	Standard
Product Type	Crosslinker
Spacer	Long (>30 Å)
Unit Size	Each

FAQs

Question: What reactive groups are present?

Answer:The crosslinker contains an NHS ester for amines and a maleimide for sulfhydryls.

Question: What quantity is supplied?

Answer:This configuration contains 100 mg.

Question: What is the spacer length?

Answer:The PEG8 spacer arm is approximately 39.2 Å long.

Application Note

Pierce™ SM(PEG)8 is a PEGylated heterobifunctional crosslinker that couples primary amines to sulfhydryl-containing molecules. This configuration contains 100 mg of solid reagent with a molecular weight of 689.71 g/mol. One end contains an NHS ester that reacts with primary amines, while the maleimide group reacts with sulfhydryls. The discrete PEG8 spacer is approximately 39.2 Å long and increases water solubility compared with traditional hydrophobic SMCC. The reagent is non-cleavable and is used for protein-protein conjugation, antibody labeling, surface modification, and preparation of biomolecule conjugates where a defined hydrophilic spacer is useful. It is supplied for research use.

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