Description
Quantity: 25 g
Unit Size: Each
Thermo Scientific™ Pierce Hydroxylamine-HCl has many uses, which include cleaving Asn-Gly peptide bonds and deprotecting (exposing) sulfhydryls on SATA-modified molecules for conjugation. Hydroxylamine·HCl is a strong reducing agent that is useful in biochemical crosslinking applications, including the deacetylation of SATA and chemical cleavage of EGS and Sulfo-EGS. Hydroxylamine converts carbonyl compounds (aldehydes and ketones) to their oxime derivatives in the presence of a weak base. Therefore, crosslinkers and other compounds that contain a carbonyl within their structure are cleavable with hydroxylamine-HCl.Highlights:
• Exposes protected sulfhydryl groups on SATA-modified molecules
• Cleaves carbonyl-containing crosslinkers such as EGSReagent Properties:
• Chemical name: Hydroxylamine Hydrochloride
• Chemical formula: NH_2OH·HCl
• Molecular weight: 69.49
• CAS Number: 5470-11-1
• Form: White crystals, free of foreign material
• Solubility: 10%