Thermo Fisher, 29129, Pierce™ Biotin

Name: Pierce™ Biotin
Brand: Thermo Fisher
SKU: 29129

Quantity: 1 g, Unit Size: Each, Thermo Scientific™ Pierce Biotinylated Proteins include biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and fluorescein (B-FITC) for use as controls or signal amplification in IHC via avidin-biotin complex (ABC) techniques.

Catalog Number: 29129

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Category

Phosphorylation Reagents

TagsProtein Analysis Reagents Streptavidin-Biotin Binding Products

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 g
Unit Size: Each
Biotinylated HRP and biotinylated AP are most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method. Biotinylated Fluorescein (FITC) cannot be used to polymerize and amplify the signal to the same degree as biotinylated enzymes because each fluor molecule is tagged with only one biotin molecule. However, the fluorescent variant of traditional ABC staining is possible with this unique molecule. Highlights:
• Biotinylated enzymes– biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and beta-galactosidase (B-bGal) for use in avidin-biotin complex (ABC) staining
• Biotinylated FITC– amplify the fluorescein signal of biotinylated primary antibodies by using avidin or streptavidin plus biotinylated fluorescein (B-FITC)
• Biotinylated controls– use biotinylated bovine serum albumin (B-BSA) or any one of these molecules as positive controls in biotin quantitation methods

Specifications

General

Description	Pierce Biotin
Label or Dye	Biotin & Analog
Product Type	Biotin (Unconjugated)
Quantity	1 g
Reactive Moiety	Carboxylic Acid
Solubility	DMSO (Dimethylsulfoxide), Water, DMF (Dimethylformamide)
Conjugate	Biotin
Form	Powder
Product Line	Pierce
Unit Size	Each

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