Description
Quantity: 50 mL
Unit Size: Each
Features of this affinity matrix include:
• Highly selective for human IgG4
• No cross-reactivity with other antibody subclasses (IgA, IgD IgE, IgM) or antibody subtypes (IgG1-3)
• No cross reactivity with other species, allowing use of complex growth media containing fetal calf or horse serum
• Excellent scalability
• Non-animal-derivedFree of animal componentsCaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).Main characteristics:
• Matrix:agarose-based, epoxide activated
• Average particle size:65 ± 10 μm
• Ligand:CaptureSelect IgG4 affinity ligand
• Ligand coupling method:epoxide coupling
• Binding capacity:>15 g IgG4/L resin depending on flow rate, column height, and contact time
• Elution conditions:0.1 M glycine, pH 3.0
• Flow characteristics:50–200 cm/h (up to 2 bar)
• Formulation buffer:20% (v/v) ethanol
