Thermo Fisher, 31497, EZ-Link™ Activated Peroxidase Antibody Labeling Kit

Quantity: 5 Reactions
Unit Size: Each
This chemistry can be used to couple HRP to primary amines on another protein such as an antibody. The Thermo Scientific Activated Peroxidase Kit is designed to purify HRP-antibody conjugates by using Immobilized Protein A/G to separate unreacted enzyme from the antibody conjugate. Using Activated HRP produces an HRP-antibody conjugate in which both the enzyme activity and the antigen-binding activity are preserved. Highlights:
• Reacts with primary amines to form a covalent amide bond
• No reduction step is necessary to secure the linkage
• Can be stored for at least 12 months at -20'C
• One mg produces about 0.5mL of conjugate with a working dilution of 1:1,000 when coupled to a high titer antibody
• Enzyme activity is > 200 units/mg Includes:5mg Activated Peroxidase, 50mL Conjugation Buffer, 250mg Lysine, 0.5mL Immobilized Protein A/G Column, 200mL Gentle Ag/Ab Binding Buffer, 200mL Gentle Ag/Ab Elution Buffer