Thermo Fisher, 4337450, BigDye™ Terminator v1.1 Cycle Sequencing Kit, 100 Reactions

Name: BigDye™ Terminator v1.1 Cycle Sequencing Kit, 100 Reactions
Brand: Thermo Fisher
SKU: 4337450

Quantity: 100 Reactions, Unit Size: Each

Catalog Number: 4337450

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Category

Sequencing Reaction Kits

Pack Size100 reactions

TagsDNA Sequencing Biochemical Reagents DNA Reagents

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 Reactions
Unit Size: Each
This BigDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.
• Improve the quality of your results for a wide range of sequencing applications
• Sequence challenging templates and sequences more successfully
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costsTackle Tough SequencesWith the BigDye Terminator v1.1 Cycle Sequencing Kit you get a robust, highly flexible chemistry for a wide range of applications, including de novo sequencing and resequencing.Get More Uniform Peak Heights, Improved AccuracyWith BigDye Terminator v1.1 kit's superior chemistry, you generate data with uniform peak heights and optimized signal balance-which results in longer, higher-quality reads. You also get more accurate base assignments for heterozygote and mutation detection.Easily Integrate into Your WorkflowBigDye™ Terminator v.1.1 kit's chemistry requires no new software or instrument recalibration.You can easily integrate either kit into your current workflow with minimal changes to your protocols.

Specifications

General

For Use With (Application)	Standard Sequencing
For Use With (Equipment)	SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐Well and 384‐Well Thermal Cyclers
Product Line	BigDye™ Terminator
Product Type	Sequencing Buffer
Quantity	100 Reactions
Technique	Fluorescent Dye Terminator Sequencing
Template Compatibility	BAC DNA, Plasmid DNA (≤15 Kb), PCR Amplicons, Single Stranded DNA
Format	96- or 384-well Plate
Unit Size	Each

FAQs

Question: What applications particularly benefit from BigDye Terminator v1.1 chemistry?

Answer:It is well suited to short PCR templates and sequencing that requires strong base calling close to the primer.

Question: How many sequencing reactions are supported?

Answer:This configuration provides reagents for 100 reactions.

Question: What analysis method is used after cycle sequencing?

Answer:The extension products are separated and detected on compatible capillary-electrophoresis genetic analyzers.

Application Note

BigDye™ Terminator v1.1 Cycle Sequencing Kit is used for fluorescent dye-terminator Sanger sequencing when high-quality base calling is required close to the sequencing primer or when short PCR-product templates are analyzed. The v1.1 chemistry is particularly suited to applications where sequence information near the primer is important and can be used with plasmid DNA, single-stranded DNA, BAC DNA and PCR amplicons. This configuration provides reagents for 100 sequencing reactions and includes BigDye Terminator v1.1 Ready Reaction Mix, M13 control primer, pREF-BDT control DNA and 5X sequencing buffer. Reaction products are analyzed by compatible capillary-electrophoresis genetic analyzers. The kit supports routine standard sequencing while offering a different dye-terminator chemistry profile from v3.1 for applications that benefit from short-read and near-primer performance.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
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