Thermo Fisher, 4404312, Sequencing Standard, BigDye™ Terminator v3.1, for 3500/SeqStudio™ Flex

Sequencing Standard, BigDye™ Terminator v3.1, for 3500/SeqStudio™ Flex is designed for spectral calibration, performance checking and control sequencing on Applied Biosystems 3500 and SeqStudio Flex series genetic analyzers. The kit contains four tubes, and each tube contains lyophilized DNA of a known 1200-base-pair sequence that has been prepared with BigDye Terminator v3.1 chemistry. One tube provides enough standard for one spectral calibration or performance check and can also be used for a control sequencing run. Lyophilization improves stability during storage and provides a defined reference sample for evaluating sequencing performance. Because the DNA sequence and dye-terminator chemistry are controlled, the standard can be used to verify that capillary separation, fluorescence detection, spectral separation and base-calling conditions are working as expected after installation, maintenance or calibration. The product is intended for use with 3500 and SeqStudio Flex series genetic analyzers and supports routine quality checks within Sanger sequencing laboratories. Using a prepared sequencing standard reduces variability associated with generating an in-house reference sample and provides a consistent material for comparing instrument performance over time. Each tube provides a controlled reference generated with the same fluorescent terminator chemistry used in BigDye v3.1 sequencing. This makes the material useful when checking whether apparent changes in read quality arise from the instrument and spectral system rather than from an unknown experimental template.