Thermo Fisher, 4481972, TaqMan™ P35S Maize Quantification Kit

The TaqMan™ P35S Maize Quantification Kit is a real-time PCR assay for determining the amount of P35S promoter sequence relative to endogenous maize DNA in food and feed samples. This configuration supports 48 reactions. Separate qPCR systems quantify the transgenic P35S target and the maize reference target, allowing the proportion of P35S-containing material to be calculated from standard curves. TaqMan MGB probe chemistry supports detection of short DNA targets, which is useful for processed foods where DNA can be fragmented. The assay has a reported quantification limit of about 20 DNA copies and a detection limit of approximately three copies for the maize and P35S systems. The kit is intended for food-quality and GMO-testing workflows using compatible real-time PCR instruments.