Description
Quantity: 200 μL
Unit Size: Each
CaptureSelect KappaXP matrix recognizes the kappa light-chain of human antibodies, enabling purification of all Kappa containing Ig's, Fab, and Fab2 fragments directly from complex sources and feed streams.Features of this affinity matrix include
• Purification of Kappa monoclonal antibodies, Fab, and Fab2 fragments in a single step with high purity and yield
• Mild elution for Fab fragments and antibodies
• Human-specific, no binding to bovine antibodies
• Excellent scalability
• Excellent pH stability up to 100 mM NaOH (limited exposure)
• Non-animal derivedFree of animal componentsCaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).Characteristics
• Column size:200 μL, 1 strip, 8 columns (8-row)
• Column dimension:5-mm diameter, 10-mm bed height
• Matrix:agarose based, epoxide-activated
• Average particle size:65 ± 10 μm
• Ligand:CaptureSelect KappaXP affinity ligand
• Ligand coupling method:epoxide coupling
• Fab binding capacity:20–30 g IgG Kappa-Fab/liter resin depending on flow rate, column height, and residence time
• IgG binding capacity:35–45 g IgG/liter resin depending on flow rate, column height, and residence time
• Elution conditions:20 mM citric or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl_2, pH6
• Formulation buffer:20% (v/v) ethanol
• Max operating pressure:2 bar
• Flow rate:16–150 cm/hrRoboColumn is a trademark of Repligen.
