Description
Quantity: 5 mL
Unit Size: Each
Features of this affinity matrix include:
• Single-step purification of immunogloblulins, Fab, and Fab2 fragments directly from complex source materials
• Mild elution for Fab fragments and antibodies
• Elution possible at pH 6.0 with 1.5 M MgCl_2
• Excellent pH stability up to 100 mM NaOH for limited exposure
• Specific for human antibodies, works in the presence of serum (bovine/horse)
• Non-animal-derivedFree of animal componentsCaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).Main characteristicsMatrix: agarose-based, epoxide-activatedAverage particle size: 65 ± 10 μmLigand: CaptureSelect KappaXP affinity ligandFab binding capacity: 20–30 g IgG Kappa-Fab/liter resin, depending on residence timeIgG binding capacity: 35–45 g IgG/liter resin, depending on residence timeElution conditions: 20 mM citric acid or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl_2, pH 6Column dimensions: 7 x 25 mm (1 mL column); 16 x 25 mm (5 mL column)Max. recommended flow: 5 mL/min. (1 mL column); 10 mL/min. (5 mL column)Max. operation pressure: 2 barFormulation buffer: 20% (v/v) ethanol