Description
Quantity: 5 mL
Unit Size: 5 mL
Features of this affinity matrix include:
• Platform approach for purification of IgGs and their Fab fragments directly from complex source materials in a single step with high purity and yield
• Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
• No co-purification of overproduced free light chains or light chain dimers
• Specific for human antibodies; works in the presence of serum (bovine/horse)
• Non-animal-derivedFree of animal componentsCaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created using a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).Main characteristics:Matrix: agarose-based, epoxide-activatedAverage particle size: 65 ± 10 μmLigand: CaptureSelect CH1-XL affinity ligandLigand coupling method: epoxide couplingBinding capacity: >15 mg/mL Fab's depending on flow rate, column height, contact timeElution conditions: 50 mM sodium acetate buffer, pH 4.0–4.5Column dimensions: 1 mL column: 7 x 25 mm; 5 mL column: 16 x 25 mmMax. recommended flow: 1 mL column: 6 mL/min; 5 mL column: 10 mL/minMax. operation pressure: 2 barFormulation buffer: 20% (v/v) ethanol
