Description
Quantity: 5 mL
Unit Size: Each
Features of this affinity matrix include:
• High recovery and purity of the target protein directly in a single step
• Mild elution, to retain biological activity and prevent aggregation of Fc fusion proteins and IgG
• Specific for human antibodies; works in the presence of serum (bovine/horse)
• Non-animal-derivedFree of animal componentsCaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).Main characteristicsMatrix: agarose-based, epoxide-activatedAverage particle size: 65 ± 10 μmLigand: CaptureSelect FcXP affinity ligandBinding capacity: 40–50 mg IgG/mL, depending on residence timeElution conditions: 20 mM citric acid or acetic acid, pH 3–4,or 100 mM Tris, 2.0 M MgCl_2, 40% (v/v) propylene glycol, pH 7Column dimensions: 7 x 25 mm (1 mL column); 16 x 25 mm (5 mL column)Max. recommended flow: 5 mL/min. (1 mL column); 10 mL/min. (5 mL column)Max. operation pressure: 2 barFormulation buffer: 20% (v/v) ethanol