Description
Quantity: 100 μL
Unit Size: Each
CaptureSelect HRP Anti-AAVX Conjugate can be combined with CaptureSelect Biotin Anti-AAVX Conjugate (Cat. No. 7103522100) in enzyme linked immunoassays to quantify the total number of AAV capsids. Sandwich ELISAs can be designed using the biotin anti-AAVX conjugate as a capture reagent immobilized on a streptavidin coated surface. HRP anti-AAVX conjugate is then used for detection of the bound AAV capsids. The unique binding properties of this pair of anti-AAVX conjugates can circumvent the need for incorporation of different AAV serotype-specific antibodies for AAV assay development.For assay development to measure AAV9 total capsids, we recommend CaptureSelect Biotin Anti-AAV9 Conjugate (Cat. No. 7103332100) and CaptureSelect HRP Anti-AAV9 Conjugate (Cat. No. 7303332100).Product details
• Format type:HRP, liquid
• Buffer:PBS, 50% (v/v) glycerol
• Storage:in freezer (-5 to -30°C)
• Concentration:0.5 mg/mL
• Volume:0.1 mL
• Recommended working dilution for sandwich ELISA:1: 5000 to 1: 10,000 (the optimal working dilution should be determined empirically for each application)Free of animal componentsCaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
