Description
Quantity: 5 mL
Unit Size: Each
Trialkylphosphines serve as excellent agents for the reduction of disulfide bonds in proteins, peptides and other disulfide bond-containing molecules and are relatively unreactive toward other functional groups. The trialkylphosphine TCEP is stable in aqueous solutions and does not undergo the rapid oxidation that often occurs with other reducing agents such as dithiotreitol (DTT) and β-mercaptoethanol (BME). TCEP does not interfere with common sulfhydryl-reactive reagents (e.g., maleimide crosslinkers). Nevertheless, many protocols require recovery of the reduced sample separate from the reducing agent. Immobilized TCEP Disulfide Reducing Gel eliminates the need to use laborious and inefficient gel filtration methods to separate the reduced sample from the reducing agent. Immobilized TCEP Disulfide Reducing Gel may be adapted conveniently to a variety of scales and formats (i.e. batch, spin column and gravity-flow column procedures). Highlights:
• Odorless: reduce proteins at your desktop; contributes to a healthier lab environment
• Stable: virtually no loss of activity when stored refrigerated
• Versatile: reduces peptides and proteins over a broad range of pH, salt, detergent and temperature conditions
• Flexible: resin slurry provides flexibility for use in spin tube, column or batch modes
• Time-saving: no soluble reductant removal saves time and conserves sample
• Efficient: reverses both natural disulfide bonds and disulfide bonds artificially incorporated by crosslinking; excellent alternative for uncoupling crosslinked proteins or peptides by agents such as DSP and DTSSP
