Description
Quantity: 10 Columns
Unit Size: Each
The GlycoLink Kits use hydrazide-activated resin and a special catalyst to prepare affinity columns with polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions. Any glycosylated proteins (even monoclonal antibodies) that contain periodate-oxidizable carbohydrate groups can be immobilized with these kits to make reusable affinity columns. Antibodies immobilized by this hydrazide immobilization chemistry have unobstructed antigen-binding sites and optimal purification capability. Prepared columns with stable glycoproteins or antibodies can be regenerated and reused at least five times for affinity-purification without significant loss in binding capacity. Highlights:
• High-capacity– immobilize 1 to 10mg of oxidized antibody or other glycoprotein per milliliter of resin
• Fast and efficient– immobilize in as few as 30 minutes; achieve at least 90% coupling of most glycoproteins in less than 4 hours (efficiency depends on the amount and type of glycosylation)
• Specific immobilization– the hydrazide-activated UltraLink Resin conjugates only to purified glycoproteins whose sugar (e.g., sialic acid) groups have been gently oxidized with periodate
• Stable attachment– resonance structure of the hydrazone bonds are sufficiently stable for multiple affinity purifications with one batch of prepared resin; no stabilization step is required
• Preserves antibody function– immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target
• Convenient sizes– choose from two complete 10-column kits: one with 1mL columns and one with 0.1mL columns UltraLink Hydrazide Resin:
• Ligand: hydrazide
• Support: Thermo Scientific UltraLink Biosupport
• Spacer arm: 22 atoms
• Loading: activated with at least 15μmol hydrazide per milliliter of settled resin
• Format: 50% resin slurry in 0.02% sodium azide Includes:Kits contain GlycoLink Columns; coupling buffer, coupling catalyst and wash buffer; oxidizing reagent; desalting columns, PBS buffer pack; and column accessories Recommended for:Purify antigens using covalently coupled antibodies; Determine binding partners for a glycoprotein of interest; Perform large-scale affinity purifications
