Thermo Fisher, 88944, GlycoLink™ Coupling Catalyst

Quantity: 100 mL
Unit Size: Each
The GlycoLink Coupling Catalyst is a two-component kit composed of aniline and sodium acetate buffer. The catalyst accelerates the coupling rate of hydrazide and alkoxyamine moieties with reactive aldehydes that have been generated by periodate-oxidation of cis-diols in glycoproteins. The GlycoLink Coupling Catalyst increases the coupling efficiency of these reactions, decreasing the molar excess amounts of labeling reagents that are required for conjugation reactions. The catalyst is also useful for increasing the binding efficiency of oxidized glycoproteins or other carbohydrate-containing molecules to hydrazide resins.Highlights:
• Optimized– catalyzes aldehyde-hydrazide and aldehyde-alkoxyamine conjugation reaction
• Efficient– maximizes coupling efficiency and decreases required coupling reaction incubation time
• Gentle– does not interfere with protein function and is easily removed by desalting or dialysis Recommended for:Labeling aldehyde-containing molecules with alkoxyamine-biotins; Labeling aldehyde-containing molecules with hydrazide-biotins; Increase coupling efficiency of aldehyde-containing molecules to hydrazide resin