Thermo Fisher, A1083708, RiboMinus™ Eukaryote Kit for RNA-Seq

Quantity: 8 Preps (10 μg⁄prep)
Unit Size: Each
The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA• Rapid and efficient removal of rRNA-probe complex using magnetic bead technologyThree Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module (Cat. No. K155005).