Thermo Fisher, A11141, ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System

Name: ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System
Brand: Thermo Fisher
SKU: A11141

Quantity: 20 Reactions, Unit Size: Each

Catalog Number: A11141

Pricing

Send an RFQ.

View RFQ List

Category

Liver Function Reagents

Pack Size20 reactions

TagsTransfection Reagents Biochemical Reagents Nucleic Acid Introduction Reagents

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 20 Reactions
Unit Size: Each
The ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen's ViraPower™ HiPerform™ Lentiviral, T-REx™ and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based, regulated (Tetracycline-inducible), high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3/ TO/V5-DEST vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.Advantages
• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway™ technology
• Stable, long-term, tetracycline-regulated expression
• Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systemsKey Features
• WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepatitis virus, increases transgene expression and cPPT (central Polypurine Tract) from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
• Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene
• Blasticidin selection marker for stable selection under control of SV40 promoterKit includes
• ViraPower™ HiPerform™ T-Rex™ Gateway™ Vector Kit (Cat. No. A11144)
• ViraPower™ Bsd Lentiviral Support Kit (K497000)
• 293FT Cell Line (R70007)
• Gateway™ LR Clonase™ II Plus Enzyme Mix (12538120)
• One Shot™ Stbl3™ Chemically Competent E. coli (C737303)
• Geneticin™ (10131035)
• pENTR™ Gus positive control plasmid

Specifications

General

Antibiotic Resistance Bacterial	Ampicillin (AmpR), Blasticidin (BsdR)
Cloning Method	Gateway
Constitutive or Inducible System	Inducible
Delivery Type	Lentiviral
For Use With (Application)	Protein Expression
For Use With (Equipment)	Gateway™, HiPerform™, T-REx™, ViraPower™
Gateway Enzyme	LR Clonase™ II Plus, LR Clonase™ II
Inducing Agent	Doxycycline, Tetracycline
Key Functions	Viral Expression
No. of Reactions	20 Reactions
PCR Enzyme	Proof-Reading Polymerase
Product Line	Gateway, HiPerform, T-REx, ViraPower
Product Type	Mammalian Expression system
Protein Tag	V5 Epitope Tag
Quantity	20 Reactions
Replication Origin	pUC Origin
Selection Agent (Eukaryotic)	Blasticidin
Selection Marker Eukaryotic	BsdR
Selection Marker Promoter	PGK Promoter
Special Elements	WPRE, cPPT
Cell Type	293-FT
Format	Kit
Promoter	CMV/TO

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.