Thermo Fisher, A16518, CytoTune™-iPS 2.0 Sendai Reprogramming Kit

Name: CytoTune™-iPS 2.0 Sendai Reprogramming Kit
Brand: Thermo Fisher
SKU: A16518

Quantity: 3 Pk., Unit Size: Each, The CytoTune-iPS 2.0 Sendai Reprogramming Kit improves upon the well-referenced CytoTune-iPS Sendai Reprogramming Kit. This system continues to be easy to use and works with many different somatic cell types, using Sendai particles to deliver Yamanaka factors, which have been shown to be critical for efficient generation of iPSCs. This kit includes three vector preparations: polycistronic Klf4–Oct3/4–Sox2, cMyc, and Klf4.

Catalog Number: A16518

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Category

Viral Transduction Reagents

TagsBiochemical Reagents Transcription and Translation Reagents

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 3 Pk.
Unit Size: Each
Features include:
• Ease of use— still requires only one application of the virus to obtain successful reprogramming
• Increased efficiency—usually at least two-fold higher reprogramming efficiencies than the original kit
• Rapid clearance—use the non-integrating Sendai virus that remains in the cytoplasm, reducing the likelihood of integration into the host genome. With the CytoTune-iPS 2.0 Sendai virus, the Sendai backbone and transgenes clear the cytoplasm faster than the original.

Specifications

General

Delivery Type	Sendai virus
For Use With (Application)	Stem Cell Engineering & Reprogramming
Product Line	CytoTune
Product Type	Sendai Reprogramming Kit
Promoter	Proprietary
Quantity	3 Pk.
Shipping Condition	Dry Ice
Sub Cellular Localization	Cytoplasm
Cell Type	iPSC
Format	Kit
Unit Size	Each

FAQs

Question: What reprogramming factors are delivered by this kit?

Answer:The kit delivers Oct3/4, Sox2, Klf4 and c-Myc using three Sendai vector preparations.

Question: What does the 3-pack configuration contain?

Answer:It provides three 100 µL vials of each of the three vector preparations.

Question: Does the Sendai system integrate into the host genome?

Answer:No. It is a non-integrating viral reprogramming system.

Application Note

CytoTune™-iPS 2.0 is a non-integrating Sendai-virus reprogramming system for converting somatic cells into induced pluripotent stem cells. The kit delivers the four Yamanaka factors through three vector preparations: a polycistronic KOS vector expressing Klf4, Oct3/4 and Sox2, plus separate c-Myc and Klf4 vectors. This A16518 configuration is the three-pack format, providing three 100 µL vials of each vector preparation. The vectors are supplied at a lot-dependent infectious titer and are stored at −80 °C. The system is designed for a single vector application during reprogramming and is used with appropriate cell-culture media and matrix conditions for the selected somatic cell type. Because the vectors are replication-defective but biologically active, reprogramming work requires appropriate biosafety practices and controlled cell-culture handling. Clearance of Sendai vector RNA should be confirmed during iPSC characterization when the downstream workflow requires transgene-free reprogrammed cells.

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Unless otherwise stated, the minimum order amount is USD 1,000.
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