Thermo Fisher, A26217, Ion Universal Library Quantitation Kit

Quantity: 250 Reactions
Unit Size: Each
The Ion Universal Library Quantitation Kit enables:
• Precision—TaqMan™ assays enable greater specificity and accuracy in detecting properly adapted, amplifiable templates over non-probe-based quantitation methods
• Sensitivity—the protocol supports quantitation of femtomolar amounts of fragment libraries
• Simplicity—the validated, ready-to-use qPCR standard requires only a simple serial dilution to determine the optimal concentration of library to use for templated bead preparation
• Versatility—the highly robust qPCR mix accommodates a wide range of cycling conditions and reaction volumes, and combines highly sensitive detection with a broad quantification range
• Efficiency—qPCR-based methods use less material for quantificationThis kit includes:
• A TaqMan™ assay designed for Ion library adaptors
• TaqMan™ Universal PCR Master Mix
• An adapted E. coli DH10B library control of a defined size constructed using the Ion Fragment Library Kit to yield an adapted library ∼190 bp in sizeNote: In addition to use with the Ion 16S™ Metagenomics Kit workflow, the Ion Universal Library Quantitation kit is also compatible with sequencing workflows for amplicons generated using any master mix.