Thermo Fisher, A39250, Expi293™ GnTI- Expression System Kit

Quantity: 1 kit
Unit Size: Each
System kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity and therefore lack complex N-glycans. The cell line was established by the selective knockout of the GnTI gene in parental Expi293F cells. This cell line is a powerful tool for the high-yield expression of homogeneously glycosylated recombinant proteins.Features:
• Homogeneous N-glycosylation of expressed proteins
• Equivalent protein yields to parental Expi293F cells - up to 1 g/L
• Significantly higher protein yields than other HEK293 GnTI- cell line alternatives
• Suspension, high-density culture of Expi293F GnTI- cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancersComponents included:
• 1 vial of frozen Expi293F GnTI- cells
• 1 L of Expi293 Expression Medium
• 1 ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture
• 100 mL Opti-Plex Complexation Buffer
• 1 PNGase F Glycan Cleavage kit
• pRABBIT IgG IRES-EmGFP positive control vectorAll components of the Expi293 GnTI- Expression System are also available for individual purchase