Thermo Fisher, A50577, TrueCut™ HiFi Cas9 Protein (5 μg/μL)

Name: TrueCut™ HiFi Cas9 Protein (5 μg/μL)
Brand: Thermo Fisher
SKU: A50577

Quantity: 500 μg, Unit Size: Each, Invitrogen TrueCut HiFi Cas9 Protein is a high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency.

Catalog Number: A50577

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Category

Phosphatase Inhibitors

Pack Size500 µg

TagsModifying Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 μg
Unit Size: Each
Features include:
• Near complete elimination of off-target events in all tested cell lines including standard, immune, primary, and stem
• Maintains maximum on-target editing efficiency compared to TrueCut Cas9 Protein v2
• Transfection-ready using either electroporation or lipid-mediated transfection reagents
• Manufactured under strict ISO-13485 quality standards
• Ideal for applications that are sensitive to off-target events and when more precise editing is required
• Eliminates time-consuming cloning stepsAvailable in two Concentrations:
• 1 μg/μL: for use in standard editing scenarios
• 5 μg/μL: for optimized editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
• For custom sizes or concentrations, please inquireOptions for obtaining CRISPR gRNA for use with TrueCut Cas9 Protein v2:
• Order pre-designed TrueGuide Synthetic gRNAs for high-efficiency knock out in human or mouse
• Submit your own design for custom synthesis of CRISPR sgRNA
• Use our free TrueDesign CRISPR Genome Editor software for easy design and ordering of custom gRNA for precise genome editing

Specifications

General

Product Type	HiFi Cas9
Purity	≥90%
Quantity	500 μg
Source	E. coli
Concentration	5 μg/μL
Form	Liquid
Sensitive to Heat Inactivation	Yes
Unit Size	Each

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
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