Thermo Fisher, A50942, DDM/CHS Solution (10:1)

Quantity: 10 x 1 mL
Unit Size: Each
• A ready-to-use solution for solubilization of membrane proteins while maintaining structural integrity and activity
• The stable pre-formulated solution contains a mixture of dodecyl-β-D-maltoside (DDM) (100 mg/mL) and cholesteryl hemisuccinate (CHS) (10 mg/mL) in deionized water with final concentrations of 10% and 1%, respectively
• Typical working concentrations are 1% DDM/0.1% CHS for protein extraction and 0.1% DDM/0.01% CHS for protein purificationFeatures of DDM/CHS Solution include:
• Ready-to-use liquid formulation
• Mixture of nonionic detergent, DDM, with dual hydrophobic/hydrophilic properties and anionic detergent, CHS, that aids in the stabilization of large unilamellar vesicles
• DDM/CHS micelles aid in solubilization of membrane proteins while preserving structure and function
• Suitable for protein solubilization and stabilization for single particle cryo-EM analysisProperties of DDM:
• Molecular formula: C_24H_46O_11
• Molecular weight: 510.6 g/mol
• Detergent class: Non-ionic
• Micelle molecular weight: 50,000 g
• Critical micelle concentration (CMC): 0.17 mM (0.009% w/v) in water; 0.12 mM (0.006% w/v) in 0.2 M NaClProperties of CHS:
• Molecular formula: C_31H_50O_4· C_4H_11NO_3
• Molecular weight: 607.9 g/mol
• Detergent: Ionizable anionic
• Function: Stabilizes large unilamellar vesicles