Thermo Fisher, A65423, SuperScript™ IV Template Switching RT Master Mix, 48 reactions

No. of Reactions: 48 Reactions
Unit Size: Each
Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions. It contains SuperScript IV Reverse Transcriptase, RNase Inhibitor, and all other reverse transcription reaction components in a convenient premixed format. Only RNA template, RT primer, and template switching oligo (TSO) of choice need to be added. Features of SuperScript IV Template-Switching RT Master Mix include: • Formulated for high quality full-length cDNA synthesis • Includes proprietary enhancer to maximize template switching efficiency • Exceptional sensitivity for easy detection of low abundance targets (as low as single cell or 2 pg total RNA) • Cell lysis buffer included for direct cDNA synthesis without RNA purification Procedure overview To maximize template switching efficiency, SuperScript IV Template-Switching RT Master Mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (TdT) activity of SuperScript IV Reverse Transcriptase. When the reverse transcriptase reaches the 5’ end of the RNA template, TdT activity adds 1–3 extra nucleotides to the cDNA end, enabling binding of the template switching oligo and incorporation of known sequence at the 3’ end of the resulting cDNA. This cDNA can be used for amplification by PCR, gene expression analysis by real-time PCR, or as a template for 5’RACE, RNA-seq, or second strand cDNA synthesis.This master mix is compatible with a wide input range of total RNA (from 2 pg to 4 µg) and is supplied with lysis buffer to enable direct cDNA synthesis from mammalian cells (1–1,000 cells per sample). Related products SuperScript IV Template-Switching RT Master Mix is compatible with various RT primers and self-designed template switching oligos. We offer Template Switching Oligo (Cat. No. A65427) and Capturing Oligo (dT) Primer (A65429), both containing the same adaptor sequence complementary to Preamplification Primer (A65428). Alternatively, a full set of reagents for template switching cDNA synthesis and preamplification is available in the SuperScript IV Single Cell/Low-Input cDNA PreAmp Kit (11752048).