Thermo Fisher, AM2548, Recombinant Proteinase K Solution (20 mg/mL)

Recombinant Proteinase K Solution (20 mg/mL) is designed for proteolytic sample preparation and the removal of protein contaminants during DNA and RNA isolation. The recombinant Proteinase K is supplied as a ready-to-use liquid solution at 20 mg/mL, with five 1.25 mL tubes in this package. As a broad-specificity serine protease, it digests proteins, including endogenous nucleases that can otherwise compromise nucleic-acid recovery. Activity is retained under denaturing conditions containing SDS or urea and in the presence of chelating agents such as EDTA. The optimum pH for enzymatic activity is approximately 6.5–9.5, and the optimum digestion temperature is about 65 °C. These properties make the solution appropriate for robust lysis and digestion protocols that expose protein-rich specimens to detergents or elevated temperatures. It also supports preparation of tissue sections for in situ hybridization. The liquid is quality-tested for nuclease contamination, including absence of detectable RNase activity. Store at −20 °C and ship on dry ice. The multi-tube format provides individually accessible portions for laboratories performing repeated nucleic-acid extraction or tissue-preparation experiments.