| Product Type | TAE Buffer |
|---|---|
| Quantity | 1 L |
| Concentration | 50X |
| Unit Size | Each |
Answer:Dilute the 50X concentrate 1:50 by mixing one volume of stock with 49 volumes of water.
Answer:40 mM Tris, 20 mM acetic acid, and 1 mM EDTA.
Answer:One liter of 50X TAE electrophoresis buffer.
Thermo Scientific 50X TAE Buffer is a concentrated Tris-acetate-EDTA solution for nucleic acid separation by agarose and polyacrylamide gel electrophoresis. The 1 L bottle provides a convenient concentrate for preparing both gel casting solutions and electrophoresis running buffer. Diluting one part of the 50X stock with 49 parts of water yields the standard 1X working solution containing 40 mM Tris, 20 mM acetic acid, and 1 mM EDTA. TAE supports electrophoretic analysis of genomic DNA, large supercoiled DNA, and RNA or DNA fragments, particularly fragments longer than approximately 1,500 base pairs. The solution is filtered through a 0.22 µm membrane and is suitable for routine molecular biology workflows. TAE has a comparatively low buffering capacity, so fresh working buffer is preferable for lengthy electrophoresis runs. Store the concentrate at 15–25 °C.
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