Thermo Fisher, C202003, One Shot™ INVαF' Chemically Competent <i>E. coli</i>

Quantity: 21 x 50 μL/tube
Unit Size: Each
One Shot INVαF´ Chemically Competent E. coli are designed to stably replicate high-copy number plasmids. They allow for the preparation of high quality plasmid DNA with less carry over contaminants as compared to other popular strains such as JM109 and can achieve a transformation efficiency of >1 x 10^8 cfu/μg supercoiled plasmid.INVαF´ cells carry the F´ episome and so can be utilized to produce single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication. This strain is related to DH strains and features similar genetic traits and benefits. The lacZΔM15 marker provides α-complementation of the ß-galactosidase gene from pUC or similar vectors and can be used for blue-white screening of colonies on bacterial plates containing Bluo-Gal or X-Gal. In addition to supporting blue/white screening, recA1 and endA1 mutations increase insert stability and improve the quality of plasmid DNA.One Shot INVαF´ Chemically Competent E. coli cells offer:• Transformation efficiencies of >1 x 10^8 cfu/μg• Stability and exceptional quality of high-copy number plasmid DNAb• Blue-white screening of recombinant clones (lacZΔM15)• Reduction of homologous recombination of transformed plasmids (recA1)• Increased quality of plasmid DNA preparations (endA1)• Production and rescue of ssDNA from vectors carrying the f1 origin of replication (F´)Note: INVαF´ cells do not require IPTG to induce expression from the lac promoter.Convenient and efficient formatOne Shot INVαF´ Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one complete transformation, reducing freeze-thaw cycles and limiting money wasted on unused cells.GenotypeF´ endA1 recA1 hsdR17 (r_K^–, m_K^+) supE44 thi-1 gyrA96 relA1 φ80lacZΔM15 Δ(lacZYA-argF)U169 λ^–Genetic marker descriptionsFind the strain and format that fit your needs• A variety of DH strains are available in chemically competent and electrocompetent cell formats.• We offer other strains for ssDNA production, such as TOP10F´ (Cat. No. C303003) or electrocompetent DH12S (Cat. No. 18312017).• MultiShot formats are available for high throughput applications.