Thermo Fisher, ER0593, NotI, HC (50 U/μL)

Quantity: 1,500 units
Unit Size: Each
5'..G C▵G G C C G C...3'3'..C G C C G G▵C G...5'Conditions for 100% Activity:
• 1X Buffer O: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl_2, 100mM NaCl and 0.1mg/mL BSA
• Incubate at 37°CStorage Buffer:
• NotI is supplied in: 20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerolLigation and Recleavage:
• After 50-fold overdigestion with NotI, more than 95% of DNA fragments can be ligated and recutMethylation Effects:
• Dam: never overlaps —no effect
• Dcm: never overlaps —no effect
• CpG: completely overlaps —blocked
• EcoKI: never overlaps —no effect
• EcoBI: never overlaps —no effectDigestion of Agarose-embedded DNA:
• Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded Adenovirus-2 DNA in 16 hoursCompatible Ends:
• Bsp120I, CfrI, Eco52I Note:Assayed using pTZ19RJL2 plasmid DNA digested with BseLI. Supercoiled plasmids may require up to 5-fold more NotI for complete digestion than linear DNAs.