Thermo Fisher, FD0404, FastDigest Eco105I

Name: FastDigest Eco105I
Brand: Thermo Fisher
SKU: FD0404

Quantity: 50 μL (50 Reactions), Unit Size: Each, Lambda DNA digested with SnaBI (Eco105I), 0.7% agarose, 1 cleavage sitesFastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4

Catalog Number: FD0404

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Category

Restriction Enzymes

Pack Size50 µL

TagsRestriction Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 50 μL (50 Reactions)
Unit Size: Each
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment (Cat. No. EP0051), T4 DNA Ligase (Cat. No. EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat. No. EP0061) all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.Features• 100% activity of all FastDigest enzymes in the universal buffer• 100% buffer compatibility with downstream applications• Complete digestion in 5–15 minutes• Direct loading on gels• No star activity• 176 FastDigest enzymes availableApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern Blot• Restriction fragment length polymorphism (RFLP)• SNP analysisNote: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.For methylation sensitivity, refer to product specifications.

Specifications

General

Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Inactivation Temperature	65°C
Overhang	Blunt
Product Type	Restriction Enzyme
Quantity	50 μL (50 Reactions)
Enzyme	Eco105I
Methylation Sensitivity	CpG Methylation-Sensitive
Optimal Reaction Temperature	37°C
Product Line	FastDigest
Research Category	Traditional Cloning
Sensitive to Heat Inactivation	Yes
Type IIS RE	No
Unit Size	Each

FAQs

Question: What DNA sequence does FastDigest Eco105I recognize?

Answer:It recognizes TAC^GTA and produces blunt-ended DNA fragments.

Question: What temperature is used for digestion?

Answer:The recommended reaction temperature is 37°C.

Question: How many standard reactions are supplied?

Answer:This configuration provides approximately 50 reactions.

Application Note

FastDigest Eco105I is used for rapid restriction digestion of plasmid DNA, genomic DNA, viral DNA and PCR products. The enzyme recognizes the sequence TAC^GTA and cleaves to generate blunt-ended DNA fragments. It is an isoschizomer of SnaBI and BstSNI and performs optimally at 37°C in the universal FastDigest or FastDigest Green Buffer. Standard digestion can be completed in approximately 5–15 minutes, allowing single, double or multiple restriction-enzyme reactions without changing buffer systems. This configuration contains 50 µL of enzyme for approximately 50 reactions and is supplied with both colorless and green 10X FastDigest buffers. The Green Buffer contains loading dyes for direct agarose-gel loading, while the colorless buffer is useful when downstream fluorescence measurements could be affected by tracking dyes.

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