Thermo Fisher, J61006.K7, TRIS-glycine-SDS running buffer (10X), pH 8.5

Quantity: 4 L
Unit Size: Each
General Description• TRIS-glycine-SDS running buffer (10X), is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteinsApplication• This solution is the most commonly used buffer for protein gel analysis in several research studies• It is also used to block membranes with 5% non-fat dry milk for western blotting• TRIS-glycine-SDS running buffer is commonly used as both anode buffer and cathode buffer• The use of SDS in the buffer helps keep the proteins linear. Glycine is an amino acid whose charge state plays an important role in the stacking gel