Thermo Fisher, J61061.MC, Deoxyribonuclease I, bovine pancreas, Purified

Quantity: 100 mg
Unit Size: Each
General Description
• Deoxyribonuclease I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5'-phosphates. In the presence of Mg2+, this compound cleaves each strand of DNA independentlyApplication
• In biomedical research, this compound is used to nick DNA as a first step to integrate labeled bases into DNA
• It can be used to eliminate genomic DNA from RNA solutions before RT-PCR
• It is also used during isolation and purification of plasma membrane vesicles from Neurospora crassa cell culture
• It can decrease viscosity in cell culture lysates, providing better yields in primary cell isolation
• It is also commonly used in combination with trypsin for the preparation of single cell suspension from rats
• Deoxyribonuclease I, bovine pancreas has been used in some studies in order to compare different methods for reducing RNase contamination in preparations of DNase
• This compound can be applied in studies to investigate the impact of the composition of sodium dodecyl sulfate (SDS) preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis