Thermo Fisher, J61679.06, Pepsin, porcine stomach

Quantity: 5 g
Unit Size: Each
General Description
• Pepsin hydrolyzes peptide bonds, but does not have the ability to hydrolyze amide or ester linkages. This compound cleaves peptides with an aromatic acid on either side of the peptide bondApplication
• Pepsin from the porcine stomach is used to form Fab fragments from antibodies and prepare F(ab)2 fragments from IgG
• It is applied to hydrolyze dry cervical samples in mice
• In biochemical studies, it has been used to demonstrate the effects of fixation and enzymatic digestion in immunohistochemical assays using paraffin-embedded tissue
• It is also used for digestion (before using immunoperoxidase techniques) to reduce non-specific background staining in sections of bronchial tissues
• This compound catalyzes the hydrolysis of aminoacyl-proline to proline