Description
Quantity: 50 Preps
Unit Size: Each
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis. Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis. RNA yields vary between different species, tissues, and age of tissue sample. See Table 1 for typical total RNA yields from various sources.Highlights
• Fast—procedure completed in 20 min following cell lysis
• Convinient—protocol does not include clogging-prone lysate filtration step
• High yield—up to 65 μg of high purity and high integrity RNA from 50 mg plant sample
• Compatiblewith wide variety of samples (leaves, roots, sprouts and other parts of various plants)ApplicationsFast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay