Thermo Fisher, K1691, RevertAid RT Reverse Transcription Kit

Quantity: 500 reactions
Unit Size: Each
The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. The enzyme maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C.First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR. It is also ideal as a first step for second strand cDNA synthesis or linear RNA amplification. Radioactively and non-radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.Features of the RevertAid RT Kit include:• Full-length first strand cDNA up to 13 kb• Optimum reaction temperature 42°C• Complete kit includes all the components for RT reactionsApplications• First strand cDNA synthesis for RT-PCR and RT-qPCR• Construction of full length cDNA libraries• Antisense RNA synthesis