Thermo Fisher, K20001, Champion™ pET200 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent <i>E. coli</i>

Quantity: 20 reactions
Unit Size: Each
• Based on T7 expression vectors originally developed by Studier and colleagues, high-level expression is achieved because the T7 RNA polymerase is more processive than nativeE. coliRNA polymerase and is dedicated to transcription of gene of interest
• Protein production is further enhanced in system by expression strain BL21 StarE. coli, which significantly improves stability of mRNA transcripts and increases protein expression up to ten-fold
• Simplified, efficient Directional TOPO cloning allows to quickly enter a ChampionpET Expression vector
• These kits feature linearized, topoisomerase I-activated ChampionpET expression vectors for five-minute directional cloning to facilitate gene expression with proofreading enzyme used for PCR, resulting in fewer errors in cloned genes
• Greater than 90% of clones are in correct orientation for gene expression, reducing the time spent on colony screeningBacterial ExpressionProtein ExpressionProteins, Expression, Isolation and Analysis