Thermo Fisher, K37001, pBAD/TOPO™ ThioFusion™ Expression Kit

Quantity: 20 reactions
Unit Size: Each
• pBAD/Thio-TOPO vector encodes His-Patch thioredoxin as N-terminal fusion partner
• Thioredoxin fusion can significantly increase solubility of many difficult-to-express proteins and improve yield of protein production
• Vector featuresaraBADpromoter for tightly regulated expression inE. coliI-activated vector for 5-minute TOPO Cloning ofTaq-amplified PCR products
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with aAnti-His(C-term) Antibody
• C-terminal V5 epitope for detection with an Anti-V5 Antibody
• Enterokinase cleavage site for efficient cleavage of N-terminal fusion tag with EKMaxEnterokinaseBacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis