Thermo Fisher, K480001, pcDNA™3.1/V5-His TOPO™ TA Expression Kit, 20 Reactions

Vector: TOPO-TA Vectors, pcDNA
Quantity: 20 Reactions
Unit Size: Each
• No ligase, post-PCR procedures, or PCR primers containing specific sequences are required
• Once cloned, analyzed, and transfected, PCR product will express directly in mammalian cell lines
• pcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-level, constitutive expression
• C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody
• C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with Anti-His (C-term)antibody
• Topoisomerase activation of pcDNA3.1/V5-His-TOPO vector allows PCR products to be ligated in 5 minutes on bench top and results in 90% recombinants
• Vector features strong CMV promoter for high-level, constitutive expression
• C-terminal V5 epitope tag for efficient detection of recombinant proteins with Anti-V5 antibodyConstitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis