| For Use With (Application) | Transfection |
|---|---|
| Product Line | Lipofectamine |
| Product Type | Transfection Reagent |
| Quantity | 0.75 mL |
| Serum Compatible | Yes |
| Shipping Condition | Room Temperature |
| Cell Type | Hard-to-Transfect Cells |
| Format | 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks |
| Sample Type | mRNA |
| Transfection Technique | Lipid-based Transfection |
| Unit Size | Each |
Answer:It is optimized for delivery of mRNA into cultured cells.
Answer:This product contains 0.75 mL of transfection reagent.
Answer:Store it refrigerated at 2–8°C and do not freeze.
Lipofectamine™ MessengerMAX™ Transfection Reagent is designed for lipid-mediated delivery of messenger RNA into cultured mammalian cells. The 0.75 mL configuration supports mRNA expression studies in established cell lines, primary cells, neuronal cells, stem cells and other cell types that can be difficult to transfect. It forms mRNA–lipid complexes for addition to plated cells, allowing the delivered message to be translated without the nuclear entry step required for plasmid DNA expression. The formulation is compatible with serum-containing culture conditions and supports formats ranging from multiwell plates to flasks, allowing researchers to adapt reagent and mRNA amounts to the culture scale. A standard workflow plates cells to reach approximately 70–90% confluence at transfection, prepares the mRNA–lipid complexes separately and then adds the complexes to the cells. The reagent is intended for mRNA delivery rather than routine standalone transfection of short single-stranded oligonucleotides. The liquid formulation is supplied as 0.75 mL and should be refrigerated at 2–8°C without freezing.
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